A study on prevalence of Methicillin resistant staphylococcus aureus (MRSA) & Extended spectrum beta lactamase (ESBL) among isolates of Diabetic foot ulcers patients

Authors

  • Dangudubiyam Sree Usha Assistant Professor, Department of Microbiology, Govt Medical College, Nalgonda, Telangana, India
  • Jayapradha Assistant Professor, Department of Microbiology, Sri Lakshmi Narayana Institute of Medical Sciences, Pondicherry, India
  • Vinod Professor & HOD, Department of Microbiology, Sri Venkateswara Medical College & Research Centre, Pondicherry, India

Keywords:

MRSA, Diabetic foot ulcers, MBL, beta-lactam antibiotics, Resistance.

Abstract

Aim: The present investigation mainly study to identify the prevalence of Methicillin resistant staphylococcus aureus (MRSA), Extended spectrum beta lactamase (ESBL) and inducible clindamycin resistance in Methicillin resistant Staphylococcus aureus (MRSA) among isolates. Methodology: Amongst 100 diabetic cases included in the present study most of them were in 5th and 6th decade, showing ulcers chiefly in right foot. Males were affected primarily than females in the ratio of 2:1. The study carried out in the Department of Microbiology at Sri Venkateswaraa Medical College and research centre from June 2012 to December 2014. Results: Among the gram negative bacilli 21 were found to be ESBL producer by phenotype and those positive was compared with gold standard method PCR for the genes TEM and SHV showed 7 and 6 positive respectively. Those gram negative bacilli showing resistance for Imipenems were detected for Metallo-beta lactamase (MBL) and carabapenemase enzyme production of which 5 and 1 were positive by phenotypic method. Those 5 positive strains of MBL were compared with gold standard method PCR for VIM gene. Only one showed positive for VIM gene. Conclusion: The prevalence rate of MRSA was found to be high. ESBL and MBL producing gram negative bacterial species have a significant impact on the clinical outcome and efforts to control outbreaks of such infections. Indiscriminate use of third generation cephalosporins to treat gram negative bacterial infections is partly responsible for the emergence of resistance to beta-lactam antibiotics. Infection control measures are required to prevent the spread and reduce emergence of resistance.

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Published

2021-08-30

How to Cite

Dangudubiyam Sree Usha, Jayapradha, & Vinod. (2021). A study on prevalence of Methicillin resistant staphylococcus aureus (MRSA) & Extended spectrum beta lactamase (ESBL) among isolates of Diabetic foot ulcers patients. International Journal of Health and Clinical Research, 4(15), 299–306. Retrieved from https://www.ijhcr.com/index.php/ijhcr/article/view/2502