Study of uropathogens and their antibiotics susceptibility pattern in tertiary care hospital in central India

Abstract

Objective This study was undertaken to determine the spectrum of organism responsible for UTI and their antimicrobial susceptibility pattern in Mahaveer Institute of Medical Sciences and Research (MIMS) located in Central India. Methods The present study was carried out in Department of Microbiology from January to December 2021. Patients attending OPD and IPD with any complaint pointing towards UTI were included study. Freshly voided, clean-catch midstream urine was All plates were then incubated at 37°C aerobically for 24 hours. A significant growth is considered if the number of colony is ≥105 colony forming unit (cfu)/ml (12). Results Most common organism isolated were gram negative organisms.E.coli (51.67%), Klebsiella (14.76%),Enterococcus (11.40).E.coli showed low level resistance for Nitrofurantoin and Amikacin (7.79%).Pseudomonas showed low resistance for Nitrofurantoin, and Amikacin (12.51%)and Meropenem and Gentamicin (37.5%).Enterococcus faecalis showed low level resistance for Nitrofurantoin(11.76%), followed by High level Gentamicin (23.52.MRSA was highly resistant to Amoxycillin (75%). Candida species isolated from various clinical specimens were identified up to species level by standard mycological techniques (77.77 %) non albicans Candida species and (22.22 %) was Candida albicans. Conclusion Our study concludes E. coli as most common organism causing urinary tract infection with female predominance. Nitrofurantoin has showed better sensitivity against most organisms causing UTI so it can be used as initial agent for empirical treatment till culture reports are available. There is increasing resistance to commonly used antibiotics due to indiscriminate use of antibiotics without culture and sensitivity report.