Comparison of diagnostic modalities for diagnosis of hepatitis-C virus infection using Rapid Antigen Diagnostic Kit and ELISA

Authors

  • Jugal Kishor Agarwal Assistant Professor, Microbiology, Rama Medical College, Hapur, U.P, India
  • Anu Sharma Assistant Professor, Microbiology, Rama Medical College, Hapur, U.P, India
  • Dipty Agrawal Assistant Professor, Anaesthesia, Rama Medical College, Hapur, U.P, India
  • Matloob Ahmad Assistant Professor, Microbiology, Rama Medical College, Hapur, U.P, India
  • Zenab Naseem Tutor, Microbiology, Rama Medical College, Hapur, U.P, India
  • M. Yasmin Professor & Head, Rama Medical College, Hapur, U.P, India

Keywords:

Hepatitis C virus, Chronic hepatitis, Hepato-cellular carcinoma, Enzyme linked immuno sorbent assay (ELISA).

Abstract

Introduction: Hepatitis-C virus (HCV) causes liver disease which can cause acute & chronic hepatitis, Illness may range from asymptomatic cases to serious life long illness which may result in chronic hepatitis & hepato-cellular carcinoma. HCV is blood borne virus transmission of which is mainly parental, infection also spread by sexual contact & from vertical transmission from mother to baby. Material & Method: Study was conducted for a period of six month. Sample send for routine anti HCV antibodies were subjected to ELISA & rapid card test. Considering ELISA as gold standard, rapid card test was compared with it. Result: Out of 2295 blood samples tested on rapid card 56 samples were reactive while 2239 sample were non reactive. On further testing with ELISA 2 samples were false positive and 8 samples were detected false negative using ELISA as gold standard test. Sensitivity of rapid test was 85.7% while specificity of rapid test was found to be 99.91%. Positive predictive value (PPV) was 96% while negative predictive value (NPV) was 99.64% . P value was <0.001 which is statistically significant and in favor of ELISA. Conclusion: Present study shows that rapid tests are inferior as compared to ELISA and hence they should not be recommended in screening of blood donor and for treatment initiation.

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Published

2022-01-18

How to Cite

Jugal Kishor Agarwal, Anu Sharma, Dipty Agrawal, Matloob Ahmad, Zenab Naseem, & M. Yasmin. (2022). Comparison of diagnostic modalities for diagnosis of hepatitis-C virus infection using Rapid Antigen Diagnostic Kit and ELISA. International Journal of Health and Clinical Research, 5(3), 718–720. Retrieved from https://www.ijhcr.com/index.php/ijhcr/article/view/4799