Ferrous sulphate - Ascorbate induced DNA damage and protection by natural compounds

Authors

  • Omkar NG Associate Professor, Department of Physiology, Shridevi Institute of Medical Sciences and Research Hospital, Tumkur, Karnataka, India
  • Bhagyalakshmi V Professor, Department of Biochemistry, Rangaraya Medical College, Kakinada, Andhra Pradesh, India
  • Dinesha Ramadas Assistant Professor, CORMIL & CMPAT, Sri Adichunchanagiri College of Pharmacy and Dept. of Biochemistry, Adichunchanagiri Institute for Molecular Medicine, Adichunchanagiri University, B.G. Nagara, Karnataka, India
  • Vedamurthy Joshi Associate Professor, Dept. of Pharmaceutics, CORMIL & CMPAT, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri University, B.G. Nagara, Karnataka, India

Keywords:

Agathi seeds (Sesbania grandiflora Linn); Antioxidant activity; Free radical scavenging; DNA damage, Cytochrome C, Ferric ion; Ferrous ion.

Abstract

Objectives: The in vitro antioxidant and inhibition of Ferrous sulphate: Ascorbate induced fragmentation of DNA by crude proteins of Agathi seeds (Sesbania grandiflora Linn) evaluated using various relevant assays. Materials and methods: Assays like DPPH, Hydroxyl radicals, Ferric ion reducing, Ferrous ion chelation, Cytochrome C and Ferrous sulphate-Ascorbate complex induced DNA damage prevention studies were done to evaluate the antioxidant capacity of Agathi seed protein. Results: Agathi seeds (Sesbania grandiflora Linn) protein showed more antioxidant and free radical scavenging activity. It scavenged approximately 71% of hydroxyl and 1,1-diphenyl-2-picrylhydrazayl radicals 61%. In addition, it reduced cytochrome c and ferric ion levels, chelated ferrous ions and inhibited Ferrous sulfate:Ascorbate-induced fragmentation and sugar oxidation of DNA. Conclusion: These results establish the antioxidant potential of crude proteins of (Sesbania grandiflora Linn).

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Published

2022-01-18

How to Cite

Omkar NG, Bhagyalakshmi V, Dinesha Ramadas, & Vedamurthy Joshi. (2022). Ferrous sulphate - Ascorbate induced DNA damage and protection by natural compounds. International Journal of Health and Clinical Research, 5(3), 721–725. Retrieved from https://www.ijhcr.com/index.php/ijhcr/article/view/4800