Ferrous sulphate - Ascorbate induced DNA damage and protection by natural compounds
Keywords:Agathi seeds (Sesbania grandiflora Linn); Antioxidant activity; Free radical scavenging; DNA damage, Cytochrome C, Ferric ion; Ferrous ion.
Objectives: The in vitro antioxidant and inhibition of Ferrous sulphate: Ascorbate induced fragmentation of DNA by crude proteins of Agathi seeds (Sesbania grandiflora Linn) evaluated using various relevant assays. Materials and methods: Assays like DPPH, Hydroxyl radicals, Ferric ion reducing, Ferrous ion chelation, Cytochrome C and Ferrous sulphate-Ascorbate complex induced DNA damage prevention studies were done to evaluate the antioxidant capacity of Agathi seed protein. Results: Agathi seeds (Sesbania grandiflora Linn) protein showed more antioxidant and free radical scavenging activity. It scavenged approximately 71% of hydroxyl and 1,1-diphenyl-2-picrylhydrazayl radicals 61%. In addition, it reduced cytochrome c and ferric ion levels, chelated ferrous ions and inhibited Ferrous sulfate:Ascorbate-induced fragmentation and sugar oxidation of DNA. Conclusion: These results establish the antioxidant potential of crude proteins of (Sesbania grandiflora Linn).
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Copyright (c) 2022 Omkar NG, Bhagyalakshmi V, Dinesha Ramadas, Vedamurthy Joshi
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