Charecterisation and resistance pattern of enterococcus species isolated from different clinical specimens in Rohilkhand region

Abstract

Objective: The objective of this study was to isolate and characterize Enterococcus species on the basis of biochemical test, antibiotics susceptibility pattern, especially in regard to vancomycin resistance and high level amino glycoside resistance from various clinical specimens of patients attending the outdoor or admitted in Rohilkhand Medical College and Hospital. Methods This study includes 6572 different clinical specimens including urine, blood, pus, vaginal swab, throat swab, ascitic fluid, pleural fluid, cerebrospinal fluid and tissue pieces received for bacteriological culture in the department of microbiology. All these specimens were cultured and screened for growth of enterococci and suspected growths were isolated and identified. Results In this study, 100 Enterococci were isolated and identified. Out of 100 Enterococci isolated from various clinical specimen 56 were from urine, 33 from pus, 04 from vaginal swabs, 05 from blood cultures and 02 from body fluids (ascitic fluid). No Enterococci were isolated from catheter tip, CSF and pleural fluid. Seventy five isolates gave the biochemical tests and other characters similar to E. faecalis out of 100 isolates. Twenty isolates were identified as E. faecium, two were identified as E. avium, one each of E.durans, E. dispar and E. raffinosus. Conclusion Species identification is useful for epidemiological investigation of an outbreak and also for clinical decisions, particularly with regards to therapy as antimicrobial susceptibility differs in different species. This will not only help in initiation of effective therapy but also in the implementation of effective infection control measures.